Host-Specific <i>Bacteroides</i> Markers-Based Microbial Source Tracking in Aquaculture Areas

Origin of fecal contamination in waters from contrasted areas: stanols as Microbial Source Tracking markers.

Improving the microbiological quality of coastal and river waters relies on the development of reliable markers that are capable of determining sources of fecal pollution. Recently, a principal component analysis (PCA) method based on six stanol compounds (i.e. 5β-cholestan-3β-ol (coprostanol), 5β-cholestan-3α-ol (epicoprostanol), 24-methyl-5α-cholestan-3β-ol (campestanol), 24-ethyl-5α-cholesta...

MICROBIAL SOURCE TRACKING - FAQs

Microbial Source Tracking within your watershed can provide important information regarding the Point Source (structures, pipes, sewage outfalls) and Non-Point Source (runoff, animals) contamination present in your service area. Determination of human, ruminant and other fecal inputs into surface source or ground waters can provide a scientific basis for watershed management decisions and can a...

Microbial Source Tracking Study

Microbial Source Tracking

Microbial contamination affects a large number of water bodies in the US. Because it is not feasible to directly monitor all pathogens, it is standard practice to monitor fecal indicator bacteria (FIB), such as total and fecal coliforms, Clostridium perfringens, Escherichia coli, and fecal enterococci, in water. Epidemiological studies have established human health standards based on exposure t...

Comparison of genotypic-based microbial source tracking methods requiring a host origin database.

Microbial source tracking (MST) results, obtained using identical sample sets and pulsed field gel electrophoresis (PFGE), repetitive element PCR (rep-PCR) and ribotyping techniques were compared. These methods were performed by six investigators in analysis of duplicate, blind sets of water samples spiked with feces from five possible sources (sewage, human, dog, cow and seagull). Investigator...